Multi purpose biological microscope is the core observation platform in modern laboratories, which goes beyond the single function of basic microscopes and integrates multiple observation techniques and modular design to meet increasingly complex scientific research and diagnostic needs. Due to its versatility, this type of microscope has become an indispensable tool for multiple interdisciplinary fields such as cell biology, medical pathology, drug development, and materials science. It can be used for routine teaching demonstrations and pathological screening, as well as for cutting-edge scientific research, truly realizing the concept of “one machine for multiple purposes”, greatly improving work efficiency and the dimensionality of experimental data.