The trinocular biological microscope is an advanced scientific instrument designed specifically for microscopic imaging and digital analysis based on the standard binocular microscope. Its most prominent feature is having a third port in addition to the traditional binocular observation tube, which is typically located at the top and is specifically designed for connecting digital cameras or CMOS sensors. This design endows it with the core ability of “watching while shooting”. Researchers can comfortably observe with the naked eye in real-time through binoculars, while transmitting optical images captured by the microscope to the camera without loss through the three eye port, achieving high-resolution static photo shooting or dynamic video recording. This makes it a standard tool for scientific research, quality control, and teaching demonstrations in modern laboratories.